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IFN,-k inhibits HIV-1 integration and post-transcriptional events in vitro, but there is only limited in vivo repression of viral production Ren-Rong Tiana,1, Hong-Xiong Guob,1, Ji-Fu Weic, Chuan-Kun Yangb, Shao-Heng Hec,⇑, Jian-Hua Wanga,⇑ a Key Laboratory of Molecular Virology and Immunology, Institute Pasteur of ,Shanghai,, ,Shanghai, Institutes for Biological Sciences, Chinese Academy of ...
Mouse ,IFN,-γ, TNF-α and TGF-β1 Elisa ,Kit, were purchased from ,Shanghai, Dakewe Biotech Co., Ltd (,Shanghai,, China). Anti-HIF-1α was purchased from Novus (,Shanghai,, China). ... Supernatants were collected for HMGB-1 ,detection, by an ELISA ,kit, (,Shanghai, Xin Yu Biotech), according to the manufacturer’s instructions.
In conclusion, we found that aerosol inhalation of ,IFN,-κ plus TFF2 in combination with standard care is safe and superior to standard care alone in shortening the time up to viral RNA negative conversion in all clinical samples. In addition, the patients in experimental group had a significantly shortened CT imaging improvement time than those in control group.
Enhanced ,IFN,-γ-dependent ISGylation in human cell lines demonstrated that ,IFN,-γ treatment also upregulates enzymes of the ISGylation pathway, as described previously for type I ,IFN, . ISG15 expression is normally induced in host cells by a variety of factors, including viral infection, TLR ligands such as lipopolysaccharide and poly IC, tumor necrosis factor-α, or type I, II, and III IFNs ...
At least 23 different variants of ,IFN,-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All ,IFN,-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the am...
OBJECTIVE To explore the relationship between allergic rhinitis and ,IFN,-γ, IL-12. METHODS The expression of IL-12 was detected by in situ hybridization and enzyme-linked immunosorbent assay in 37 patients with allergic rhinitis and 12 control samples. ,IFN,-γ was detected by ELISA. RESULTS The positive ratios of IL-12 in PBMC were 0.13±0.03 and 0.77±0.15 in allergic rhinitis group and ...
26/7/2016, · Introduction. Type I interferons (IFNs) include ,IFN,-α and ,IFN,-β. Accumulating evidence suggests that type I IFNs play multifaceted roles in diseases (Trinchieri, 2010).Initially, type I IFNs were discovered as critical antiviral cytokines, and recent reports suggest that the blockade of type I ,IFN, signaling in virus-induced chronic infection could decrease immune suppression and accelerate ...
Human ,IFN,- /IL-17 ,Kit, Catalog Number ELD5219 ... ,Shanghai, PRC 200050 E-MAIL: info@RnDSystemsChina.com.cn. INTRODUCTION The Dual-Color Human ,IFN,- /IL-17 ELISpot assay is designed for the simultaneous ,detection, of ,IFN,-and IL-17 secreting cells at the single cell level and can be used to simultaneously quantitate the