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After 1 h of LM infection (MOI = 1:1 or 10:1), gentamicin was added to kill any extracellular bacteria and the cells were cultured for 4 h. Then, these cells were harvested and washed twice with PBS, and the apoptosis of neutrophils was detected with the FITC Annexin V Apoptosis ,Detection Kit, (BestBio, ,Shanghai,, China) as previously described 45.
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Enhanced ,IFN,-γ-dependent ISGylation in human cell lines demonstrated that ,IFN,-γ treatment also upregulates enzymes of the ISGylation pathway, as described previously for type I ,IFN, . ISG15 expression is normally induced in host cells by a variety of factors, including viral infection, TLR ligands such as lipopolysaccharide and poly IC, tumor necrosis factor-α, or type I, II, and III IFNs ...
Serum levels of interferon (,IFN,)-γ, IL-4 and IL-17 were determined by ELISA using ,IFN,-γ, IL-4 and IL-17 ,kits,, following the manufacturer's instructions (,Shanghai, Yuan-Long Biotechnology Co., Ltd., ,Shanghai,, China). The plasma was placed at room temperature for 20 min, until completely thawed.
This sandwich ,kit, is for the accurate quantitative ,detection, of Fish Interferon Gamma (also known as ,IFN,-G) in serum, plasma, cell culture supernates, cell lysates, tissue homogenate. Cat.No E0210Fi. Standard Curve Range: 30ng/L – 9000ng/L.
The protein levels of human Perforin, Granzyme B, ,IFN,-γ, IL-17, IL-4 or TNF-α in the supernatants of γδ T cells were examined with ELISA ,Kits, (Arigo, #ARG80173 for Perforin, #ARG80171 for Granzyme B; NeoBioscience, #EHC102 g.48 for ,IFN,-γ, #EHC170.48 for IL-17/IL-17A, #EHC006.48 for IL-4, #EHC103a.48 for TNF-α) according to the ...
Annexin V apoptosis ,detection kit, (eBioscience, San Diego, CA, USA). Briefly, podocytes plated in 6-well plates were infected with lentivirus. At Figure 1. Overexpression of IFIT1 in podocytes by lentiviral transduction. Podocytes were infected with IFIT1 expressing lentivirus …